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Objective@#To investigate the expression of interleukin (IL)-1α, IL-1β and IL-1 receptor antagonist (IL-1RN) in cervical cancer and its correlation with clinical feature and prognosis of cervical cancer.@*Methods@#The data of 4 databases were collected and analyzed on April 28, 2019. The Cancer Genome Atlas (TCGA) database was used to analyze the relationship between IL-1α, IL-1β and IL-1RN mRNA expression and prognosis in cervical cancer tissues (292 cases). The GEPIA (Gene Expression Profiling Interactive Analysis) database (http://gepia.cancer-pku.cn) was used to analyze the correlation between IL-1α, IL-1β and IL-1RN mRNA expression and prognosis in cervical cancer tissues (292 cases), the LinkedOmics database (http://www.linkedomics.org) was used to analyze IL-1α, IL-1β and IL-1RN mRNA expression in different pathological types of cervical cancer tissues (226 cervical squamous cell carcinoma tissues and 47 cervical adenocarcinoma tissues), and the STRING database (https://string-db.org) was used to analyze protein interaction networks of IL-1α and IL-1β.@*Results@#The expressions of IL-1α, IL-1β and IL-1RN mRNA in cervical cancer tissues were significantly higher than those in normal cervical tissues (1.54 vs. 0.04, 4.82 vs. 0.46 and 73.53 vs. 0.67), and there were statistical differences (P<0.01); Kaplan-Meier survival curve analysis showed that high expression of IL-1α and IL-1β mRNA suggested poor prognosis (HR=1.7 and 1.7, P=0.021 and 0.028), and IL-1RN mRNA expression was not associated with prognosis (P>0.05). The expressions of IL-1α, IL-1β and IL-1RN mRNA were correlated between any two indexes (P<0.01), and the correlation between IL-1α and IL-1β was stronger (r=0.87, P<0.01); Kaplan-Meier survival curve analysis showed that the assessment prognosis value of IL-1α combined with IL-1β was the highest (HR=2.0). The expressions of IL-1α, IL-1β and IL-1RN mRNA in cervical squamous cell carcinoma tissues were significantly higher than those in cervical adenocarcinoma tissues (6.535 vs. 2.936, 7.382 vs. 5.588 and 11.170 vs. 8.639), and there were statistical differences (P<0.01). The associated proteins with IL-1α and IL-1β included IL-1 receptor type 1 (IL-1R1), IL-1 receptor type 2 (IL-1R2), IL-10, IL-4, IL-18, IL-1 receptor accessory protein (IL1RAP), tumor necrosis factor (TNF), caspase 1 (CASP1) and IL-8; IL1R1 mRNA expression in cervical cancer tissues was significantly lower than that in normal cervical tissues (9.56 vs. 40.961), the expressions of TNF, CASP1, IL1RAP, IL-18 and IL-8 mRNA were significantly higher than those in normal cervical tissues (1.51 vs. 0.06, 33.70 vs. 12.61, 11.12 vs. 3.02, 24.59 vs. 3.54 and 20.01 vs. 0.48), and there were statistical differences (P<0.01 or <0.05); high expression of IL-8 mRNA suggested poor prognosis (HR=2.9, P<0.01).@*Conclusions@#Overexpression of IL-1α, IL-1β and IL-1RN plays an important role in the proliferation of cervical cancer. IL-1α and IL-1β can prompt the inflammatory response and prognosis of cervical cancer, and provide a theoretical basis for the early diagnosis and prognosis of cervical cancer.
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Objective@#To investigate the correlation between bone marrow suppression and the dose volume analysis of pelvic bone and provide reference for defining the parameters of bone volume-dose restriction during pelvic radiotherapy.@*Methods@#A retrospective analysis of 102 cases of cervical cancer, 53 cases of rectal cancer and 34 cases of prostate cancer in People′s Hospital of Xinjiang Uygur Autonomous Region from January 2013 to April 2016 was performed. All patients received pelvic irradiation at a dose of 50.0-50.4Gy, and a boost of 10-24 Gy was given for different types of tumors or tumor beds. Concurrent chemotherapy was carried out in patients with cervical cancer and colorectal cancer, and endocrine therapy was given to prostate cancer patients during radiotherapy. The parameters including the target bone volume, V10, V20, V30, V40, V50, and the grade of bone marrow suppression according to RTOG grading scale within 3 months after radiotherapy or 6 months post-radiotherapy were evaluated. The correlation between the irradiation bone volume and the grade of bone marrow suppression was assessed by univariate analysis. The relationship between the grade of bone marrow suppression and age, chemotherapy and radiotherapy dose was further analyzed by multivariate analysis.@*Results@#In patients with cervical, rectal and prostate cancer, the overall incidence rate of early bone marrow suppression was 77.5%, 79.2% and 70.6%, and 65.7%, 62.3% and 35.3% for ≥grade 2 bone marrow suppression, respectively. Late-stage bone marrow suppression occurred in 11 patients, grade Ⅱ in 7 cases, and the incidence rate of ≥grade 2 bone marrow suppression was 3.7%. The target bone volume in the cervical, rectal and prostate cancer groups was (746.30±27.84) cm3, (736.15±28.72) cm3 and (740.70±35.08) cm3(P=0.023), respectively. Univariate and multivariate analyses revealed that V10, V20, V30, V40 and CV10, CV20, CV30, CV40 were not significantly correlated with the grading of bone marrow suppression (all P>0.05), whereas V50 and CV50 were significantly correlated with bone marrow suppression (both P<0.05). ROC curve analysis demonstrated that V50=72.33 cm3 and CV50=9.42% were the diagnostic cut-off values for ≥grade 2 bone marrow suppression.@*Conclusions@#The incidence of bone marrow suppression is relatively high in pelvic radiotherapy, which is correlated with the irradiation bone volume and dose in the target area. At a total dose of 50 Gy, the incidence of ≥grade 2 bone marrow suppression is not associated with ≤V40 or absolute volume. Besides, it is not correlated with dose boost or whether chemotherapy is delivered, whereas it is associated with V50 and absolute volume. V50=10.23% and CV50=72.33 cm3 are the cut-off values for the incidence of ≥grade 2 bone marrow suppression.
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investigate the expression of interleukin (IL)-1α, IL-1β and IL-1 receptor antagonist (IL-1RN) in cervical cancer and its correlation with clinical feature and prognosis of cervical cancer. Methods The data of 4 databases were collected and analyzed on April 28, 2019. The Cancer Genome Atlas (TCGA) database was used to analyze the relationship between IL-1α, IL-1β and IL-1RN mRNA expression and prognosis in cervical cancer tissues (292 cases). The GEPIA (Gene Expression Profiling Interactive Analysis) database (http://gepia.cancer-pku.cn) was used to analyze the correlation between IL-1α, IL-1β and IL-1RN mRNA expression and prognosis in cervical cancer tissues (292 cases), the LinkedOmics database (http://www.linkedomics.org) was used to analyze IL-1α, IL-1β and IL-1RN mRNA expression in different pathological types of cervical cancer tissues (226 cervical squamous cell carcinoma tissues and 47 cervical adenocarcinoma tissues), and the STRING database (https://string-db.org) was used to analyze protein interaction networks of IL-1α and IL-1β. Results The expressions of IL-1α, IL-1β and IL-1RN mRNA in cervical cancer tissues were significantly higher than those in normal cervical tissues (1.54 vs. 0.04, 4.82 vs. 0.46 and 73.53 vs. 0.67), and there were statistical differences (P＜0.01); Kaplan-Meier survival curve analysis showed that high expression of IL-1α and IL-1β mRNA suggested poor prognosis (HR=1.7 and 1.7, P=0.021 and 0.028), and IL-1RN mRNA expression was not associated with prognosis (P＞0.05). The expressions of IL-1α, IL-1β and IL-1RN mRNA were correlated between any two indexes (P＜0.01), and the correlation between IL-1α and IL-1β was stronger (r=0.87, P＜0.01); Kaplan-Meier survival curve analysis showed that the assessment prognosis value of IL-1α combined with IL-1β was the highest ( HR=2.0). The expressions of IL-1α, IL-1β and IL-1RN mRNA in cervical squamous cell carcinoma tissues were significantly higher than those in cervical adenocarcinoma tissues (6.535 vs. 2.936, 7.382 vs. 5.588 and 11.170 vs. 8.639), and there were statistical differences (P＜0.01). The associated proteins with IL-1α and IL-1β included IL-1 receptor type 1 (IL-1R1), IL-1 receptor type 2 (IL-1R2), IL-10, IL-4, IL-18, IL-1 receptor accessory protein (IL1RAP), tumor necrosis factor (TNF), caspase 1 (CASP1) and IL-8; IL1R1 mRNA expression in cervical cancer tissues was significantly lower than that in normal cervical tissues (9.56 vs. 40.961), the expressions of TNF, CASP1, IL1RAP, IL-18 and IL-8 mRNA were significantly higher than those in normal cervical tissues (1.51 vs. 0.06, 33.70 vs. 12.61, 11.12 vs. 3.02, 24.59 vs. 3.54 and 20.01 vs. 0.48), and there were statistical differences (P＜0.01 or ＜0.05); high expression of IL-8 mRNA suggested poor prognosis ( HR=2.9, P＜0.01). Conclusions Overexpression of IL-1α, IL-1β and IL-1RN plays an important role in the proliferation of cervical cancer. IL-1α and IL-1β can prompt the inflammatory response and prognosis of cervical cancer, and provide a theoretical basis for the early diagnosis and prognosis of cervical cancer.
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Objective To evaluate the efficacy of dexamethasone acupoint injection combined with conventional therapy for the paitents with sudden deafness. Methods A total of 72 patients with sudden deafness who met the inclusion criteria were randomly divided into 2 groups with 36 in each group. On the basis of conventional therapy, the observation group injected dexamethasone at acupoints; control group intravenous drip combined with oral dexamethasone. The average hearing threshold and the efficacy of symptoms between the 2 groups were compared. Results After treatment, the average hearing threshold of the observation group (49.64 ± 20.10 dB vs. 70.44 ± 21.60 dB, t=4.231), and the control group (49.25 ± 20.80 dB vs. 65.64 ± 22.30 dB, t=3.225) were both significantly lower than those before treatment (P<0.05), but there was no significant difference between the two groups after treatment (P>0.05). The effective rate of tinnitus was 85.3％ (29/34) in the observation group and 73.5％ (25/34) in the control group, and there was significant difference between 2 groups (Z=-2.109, P=0.035). The effective rate of ear fullness was 92.6％ (25/27) in the observation group and 82.8％ (24/29) in the control group, and the difference between the two groups was statistically significant (Z=-2.109, P=0.035). The effective rate of dizziness in the observation group was 92.9％ (13/14), whild the control group was 69.2％ (9/13), and the difference between the two groups was statistically significant (Z=-2.136, P=0.033). After treatment, the effective rates of the patients in the observation group of ≤7 days and >7 days were 90.5％ (19/21) and 66.7％ (10/15) respectively (Z=-2.162, P=0.031), while in the control group they were 73.9％ (17/23) and 46.2％ (6/13) respectively (Z=1.991, P=0.046). Conclusions The Dexamethasone acupoint injection combined with conventional therapy can effectively improve the accompanying symptoms such as tinnitus, ear fullness and vertigo in sudden deafness.
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Objective To assess the clinical value for the clinicopathological features of microcystic elongated and fragmented(MELF)invasion in endometrial carcinoma(EEC). Methods The clinicopathological data of 108 cases of endometrial carcinoma with total hysterectomy, bilateral adnexectomy, and pelvic dissection were retrospectively analysis in Peking University People′s Hospital from April 2015 to October 2016. Twenty-five patients with endometrial carcinoma showing MELF invasion pattern were collected. We analyzed retrospectively the association of MELF pattern invasion with clinical pathology data and prognosis of the patients,partial immunohistochemical staining was implemented. MELF invasion was a special invasion pattern and characterized by microcystic, elongated, fragmented(composed of cluster cells)gland in muscular layer. Results The incidence rate was 23.1%(25/108). These patients mean age was (59.3 ± 10.9)years old. Four cases were premenopausal, and 21 were postmenopausal. Abnormal vaginal bleeding was the main clinical presentation. The lesions tend to appear adjacent to the tumor body. Sometimes, it may be appears away from the tumor body in the deep muscle layer. Lymph node metastasis were present in 5 cases(20%,5/25). Thirteen cases(52%,13/25)of them demonstrated lymph vascular space involvement(LVSI). The immunohischemical expression of ER,PR, Ki-67 and galectin-3 showing MELF invasion pattern were weaker than no showing MELF invasion pattern endometrial carcinoma, cktokeratin (CK) was showed diffuse strong positive expression, E-cadherin was moderately positive expression. All 25 cases were followed up for(23.2±5.9)months(14-33 months)after the therapy with no recurrence on metastasis. Conclusions MELF invasion pattern is a special invasion pattern in low-grade EEC. The incidence of LVSI and lymph node metastasis rate in endometrial carcinoma with MELF invasion are significantly increased. The prognosis of MELF invasion pattern may be poor.
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Objective To observe conditioned enhancement of antibody production against influenza vaccine. Methods 36 female BALB/c mice were injected with 3 μg/mouse influenza virus hemagglutinin (HA) as the unconditioned stimulus (UCS),and camphor odor was served as the conditioned stimulus (CS). After a CS/UCS pairing was made,animals were re-exposed to the CS at Weeks 6. Results Through one conditioned stimulus,the optical density of anti-HA antibody of the conditioned group (Weeks 9:0.68±0.06; Weeks 10:0.60±0.06)was significantly increased compared with the unconditioned group (Weeks 9:0.53±0.06; Weeks 10:0.48±0.04) ( P <0.01). The level of anti-HA antibody of the conditioned group was also significantly greater than other controlled groups( P <0.05). Conclusion Through a single exposure to camphor odor which was paired with immunization of influenza virus HA in a single trial learning protocol,a significant conditioned anti-HA IgG production occurred.